Stem Cells (La Jolla)

Overview

The Stem Cell Core (SCC) at Sanford-Burnham provides scientists within the community the opportunity to conduct their research devoted to human stem cells and that is conducive to collaborative partnerships. The SCC staff has extensive experience in maintenance, characterization, and differentiation of human embryonic stem cells (hESC) and the generation of non-integrated hiPSC from skin fibroblasts and lymphocytes using episomal, modified mRNA, or Sendai viral method. The SCC provides researchers with a source of various embryonic stem cell lines and carries a wide variety of validated stem cell reagents for hESC/hiPSC culture that are available for purchase. Hands-on and individual ad hoc training courses on hESC maintenance, characterization, and hiPSC generation are available for investigators and scientists. Extensive equipment for growth and characterization of stem cells is available for use in the core.

Equipment

  • Six - and 4 - foot tissue culture hoods: general maintenance and manipulation of cultures.
  • Sanyo CO2 tissue culture incubators: advanced incubator with UV decontamination system.
  • Four-foot Envirco Flow Lamina hoods: for manual manipulation of cultures.
  • Olympus CKX-41 Tissue Culture Scope: for general visualizations and basic image acquisition of cultures.
  • XVivo Hypoxia culturing system: self-contained incubation system that allows culture, manipulate and visualize cells under user-specified hypoxic or normoxic conditions.
  • MVE 1830 Eterne cryostorage system: 190 degree-vapor phase cell storage system for up to ~80,000 2 ml vials. This unit will have a back-up N2 source in addition to a 24-hour remote alarm.
  • Biogenic Solutions 2100 Controlled Rate Freezing System: advanced computer-controlled cell freezing instrument for optimal freezing rate.
  • Leica DMI 4000B Inverted Fluorescent Microscope & Camera System: this microscope will be mainly available for image acquisition and will have available both the Metavue and the standard Leica image acquisition software.
  • Olympus IX71 Inverted Fluorescent Microscope: mainly available for image acquisition and will have available both the Metavue and the standard Olympus image acquisition software.
  • Leica M165 FC fluorescent dissecting microscopes: for routine maintenance of stem cell lines that contain fluorescent reporters. One will be housed in a 4 foot EnviroCo laminar flow hood and one will be housed in the XVivo Hypoxia System.
  • Leica MZ6 Dissecting Stereomicroscopes: housed in separate EnviroCo laminar flow hoods for the routine manipulation of stem cell cultures.
  • Backman Coulter Cell Lab Quanta SC: an advanced flow cytometry with 3-color, coulter volume and side scatter analysis.
  • Beckman Coulter Spectrophotometer
  • Eppendorf Mastercycler EP Gradient PCR instrument
  • 384-well Roche Lightcycler 480 real-time PCR instrument: a newly released Lightcycler from Roche that allows one to perform reactions in either a 96- or 384- well format. The main feature, in addition to the high quality nature of Roche products, is the ease to switch from the 96- to 384- well format without having to recalibrate the instrument.
  • A computer-controlled precise laser system (LEAP): for hESC/hiPSC clone isolation, passaging, & amplification, which enables the high-volume handling of stem cell culture and hiPSC generation.

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Contact

Evan

Evan Y. Snyder, M.D., Ph.D., F.A.A.P.

Professor and Program Director, Stem Cells and Regeneration Program
Email Evan Snyder

Dr. Snyder earned his M.D. and his Ph.D. in neuroscience from the University of Pennsylvania in 1980. He completed residencies in pediatrics and neurology at Children's Hospital-Boston, Harvard Medical School and postdoctoral research at Harvard Medical School. In 1992, Dr. Snyder was appointed an instructor in neurology at Harvard Medical School and was promoted to assistant professor in 1996. He is regarded as one of the fathers of the stem cell field, having identified over 2 decades ago that cells that came to be called stem cells were a source of neural plasticity. He was the first to demonstrate that non-hematopoietic stem cells could mediate cell and gene replacement, home to injury, and perform protective, trophic, pro-regenerative, and anti-inflammatory actions. He was the first to isolate human neural stem cells. In 2003, after 23 years at Harvard, Dr. Snyder was recruited to Sanford-Burnham Medical Research Institute as professor and director of the Stem Cells and Regeneration program.


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